Introduction to High Performance Liquid Chromatography

May, 2012

West Chester University

Course Description

A practical problem solving introduction to HPLC, students will learn HPLC fundamentals, nomenclature, separation modes (normal/ reversed-phase) and solvent/column selection. In laboratory sessions students will operate commercial equipment, solve typical HPLC problems, and learn time-saving techniques. By completion of the course, students with no previous experience in HPLC should have a working knowledge of liquid chromatography. Experienced chromatographers will benefit from discussions on new trends in optimization, quantitative analysis, and instrumentation.


CostRegular30+ Days Advance Registration
Lecture and Lab
Lecture only

Course Lecture Outline
  • Fundamentals and Definitions
  • Instrumentation
  • Columns and Column Packings
  • Mobile Phase Selection
  • Problem Solving
  • Optimization
  • Chiral Separations
  • Quantitative Analysis
  • Troubleshooting
  • Method Development
  • Eluents, Polarity, Selectivity
  • Advanced Theory, Buffers, & the Effect of pH
Faculty
For More Information

William Champion, Agilent Technologies., wlchampion@gmail.com, 302-475-2466