Printable Meeting FlierJanuary Meeting Notice
Tuesday, January 13, 2009
LCMS Analysis of Glycolipids
Bob Moreau
Eastern Regional Research Center (ERRC)
United States Department of Agriculture (USDA)
Agricultural Research Service (ARS)
600 East Mermaid Lane, Wyndmoor, Pennsylvania 19038
Abstract:
Oat kernels were extracted with methanol, and glycolipid-enriched fractions were prepared using silica solid phase extraction. Using direct infusion electrospray ionization (ESI) tandem mass spectrometry (MS), high performance liquid chromatography (HPLC)-ESI-MS, and HPLC-atmospheric pressure chemical ionization (APCI)-MS, we confirmed previous reports that digalactosyldiacylglycerol (DGDG) was the most abundant glycolipid in oat kernels and confirmed a previous report of the presence of a DGDG mono-estolide in oat kernels. In the current study we also identified several additional natural galactolipid estolides: two new DGDG estolides (di- and tri-estolides), two trigalactosyldiacylglycerol (TriGDG) estolides (mono- and di-estolides), and one tetragalactosyldiacylglycerol (TetraGDG) estolide (mono-estolide). The levels of total galactolipid estolides in oat kernels were estimated to be about 29% of the total glycolipid fraction. To our knowledge, this report is the first evidence of natural di- and tri-estolides of polar lipids.
About the Speaker:
Robert Moreau was born and raised in Massachusetts. He attended Boston University, majoring in biology, and the University of South Carolina, where he obtained his Ph.D. in plant lipid biochemistry in 1979. After a 2 year postdoc at UC Davis, Bob accepted a Research Chemist position at the Eastern Regional Research Center, USDA, ARS. For the first ten years he conducted research on membrane changes and signaling during host-pathogen interactions. In 1987 he acquired his first high performance liquid chromatograph (HPLC) and began employing it to analyze lipids in many types of biological and agricultural systems. Bob developed several new analytical methods for lipids which employed several “mass” detectors for HPLC, including flame ionization detection (FID), evaporative light-scattering detection (ELSD), and charged aerosol detection (CAD). Gradually over the years Bob began collaborating with experts who used mass spectrometry. In 2001 Bob obtained an HPLC-mass spectrometer. He has enjoyed learning the technology and applying it to several interesting research areas. For the last ten years his major ARS research projects have been in the area of bioethanol. He has participated in a multidisciplinary team where his expertise has been in the area of biofuels “co-products” research, focusing on “functional lipid” co-products such as phytosterols, tocopherols and carotenoids. Bob has authored more than a hundred peer-reviewed papers, is an inventor on four patents, and is an Associate Editor for the journal Lipids.
Details:
| Location: | D'ignazio's Towne House |
| Times: | 5:00 PM Executive Committee Meeting 5:45 PM Social "Hour" 6:30 PM Dinner 7:30 PM Presentation |
| Cost: | $30 |
| Dinner Choices: | Chicken w Bread Stuffing Veal Parmesan & Spaghetti Eggplant |
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