September Meeting Notice

Tuesday, September 16, 2008

New Developments in Solvent-Less Sample Preparation Techniques

Janusz Pawliszyn

University of Waterloo, Canada

Abstract:

In this presentation, new developments in solvent-less sample preparation techniques with emphasis on Solid-Phase Micro-Extraction (SPME) and related techniques will be discussed. In cold fiber SPME, the internally-cooled, coated fiber device is temperature-controlled and used in combination with a CTC CombiPAL autosampler. Up to 10 times increase of extraction efficiencies was observed even under the limited sample temperature. The cold fiber technology facilitates faster extractions and higher sensitivities for both volatile and semi-volatile sample components in food matrices. Thin-film SPME samplers were made by using polydimethylsiloxane thin-film (membrane) as the extraction phase. This technique offers the advantage of higher surface-to-volume ratio facilitating much higher extraction rates and higher sensitivities than current SPME fibers. Kinetic calibration or equilibrium calibration with the standards in the extraction phase is used to introduce an isotopically-labeled internal standard. This approach is very useful in high sensitivity multiparameter monitoring both in situ and in-vivo. The automation of parallel solid-phase microextraction (SPME) coupled to liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been accomplished using a 96 multi-well plate format, a SPME multi-fiber device and a three-arm robotic system. Sample throughput has been drastically increased. The optimized automated SPME-LC-MS/MS platform was fully validated for the high-throughput determination of Ochratoxin A in human urine. The proposed methods allowed the automated analysis of >150-200 samples per day, while achieving excellent accuracy and precision and requiring minimal sample pre-treatment of biological fluids. Details of these newer sample preparation technologies will be covered. Co-authors are: Yong Chen, In-Yong Eom, and Ouyang Gangfeng

About the Speaker:

Dr. Pawliszyn received his B.S. and M.Sc. Chem.Eng. degrees from Technical University of Gdansk, Poland and his Ph.D. in 1982 from Southern Illinois University. He is an author of over 350 scientific publications and a book on Solid Phase Microextraction. He is a Fellow of Chemical Institute of Canada, Editor of Analytica Chimica Acta, Trends in Analytical Chemistry and a member of the Editorial Board of Journal of Separation Science, Analyst, Chemia Analityczna and Comprehensive Analytical Chemistry book series. He received the 1995 McBryde Medal, the 1996 Tswett Medal, the 1996 Hyphenated Techniques in Chromatography Award, the 1996 Caledon Award, the Jubilee Medal 1998 from the Chromatographic Society, U.K., the 2000 Maxxam Award, the 2000 Varian Lecture Award from Carleton University, the Alumni Achievement Award for 2000 from Southern Illinois University, the Humboldt Research Award for 2001, 2002 COLACRO Medal, 2003 Canada Research Chair. In 2006 he was elected to the most cited chemists by ICI and in November, 2008 he will receive the A.A. Benedetti-Pichler Award at Eastern Analytical Symposium. He presently holds the Canada Research Chair and NSERC Industrial Research Chair in New Analytical Methods and Technologies. Contact information: Tel: (519) 888-4641 Fax: (519) 746-0435; e-mail: janusz@uwaterloo.ca; Home page for research group: http://www.science.uwaterloo.ca/chemistry/pawliszyn

Details:

Location:	D'ignazio's Towne House
Times:	5:00 PM Executive Committee Meeting 5:45 PM Social "Hour" 6:30 PM Dinner 7:30 PM Presentation
Cost:	$30
Dinner Choices:	Chicken Marsala Crabcakes Eggplant Parmesan with marinara sauce

NOTICE TO STUDENTS AND FACULTY: Full-time students with valid ID may attend dinner meetings at half-price. Faculty members at colleges and universities are urged to bring one or more students to the meeting. If they do, they also can attend at half-price.