Printable Meeting FlierDecember Meeting Notice
Thursday, December 2, 2010
Tivadar Farkas: Current Challenges in Achieving State-of-the-art HPLC Performance
Tivadar Farkas
Phenomenex (Torrance, CA)
Abstract:
Recent improvements in chromatographic resolution and speed of analysis have brought column and instrument performance to the forefront of the scientific debate. Core-shell and fully porous sub-2 micrometer particle packed columns demonstrate unprecedented chromatographic efficiency. The full potential of these novel chromatographic materials may have not yet been realized given the challenges encountered in the column packing process and also in operating highly efficient columns.
In this presentation we analyze the current performance of fully porous sub-2 micrometer and of core-shell particle packed columns, and the additional significant improvements that could arise from further refinements in particle morphology and surface chemistry. Furthermore, we review the current understanding of how core-shell particle based columns work.
Next, we discuss the impact of extra-column contributions to band spreading on the performance of the best HPLC columns available today. Convincing data proves that even state-of-the art HPLC instruments can fail to provide the level of performance necessary to preserve the resolving power of the most efficient chromatographic columns. Instrument contributions to band spreading are potentially neglected due to the lack of stringent specifications on their magnitude. Shortcomings in regards of measurement accuracy, repeatability and reproducibility with instruments of the same model will be discussed. Target performance expectations will be suggested in the light of current and foreseeable advancements in HPLC column performance. It is clear that future developments in column technology may be impossible to realize with the current level of performance of HPLC instrumentation.
About the Speaker:
I received my Ph.D. degree from the University of Tennessee at Knoxville in Analytical Chemistry in 1992 under the guidance of Prof. Georges Guiochon. The topic of my thesis was the investigation of HPLC column heterogeneity with electrochemical, and with laser induced fluorescence detection, on analytical and preparative chromatographic columns.
I started my career as an analytical chemist in gas-chromatography in fragrance and food analysis in 1987. I joined Phenomenex in 1997 as a Senior Scientist and first worked on column packing technology. For several years I managed the Analytical Support and Development group within R&D, focusing on the development of novel stationary phases, column formats, and applications for both analytical and prep HPLC. Currently I am the Senior Managing Scientist in the R&D department at Phenomenex, Inc. overseeing all major research projects.
My interests include the evaluation of novel chromatographic stationary phases using a wide range of chromatographic tests standard to the industry, and the development of additional testing procedures that better reflect column behavior toward compounds of pharmacological interest.
Our most recent successful project was the development of a new line of core-shell particle based HPLC columns capable of providing excellent chromatographic resolution even when operated on conventional HPLC instrumentation. This work brought to the forefront of our preoccupations the current limitations in achieving further improvements in chromatographic performance.
Details:
| Location: | D'ignazio's Towne House |
| Times: | 5:00 PM Executive Committee Meeting 5:45 PM Social "Hour" 6:30 PM Dinner 7:30 PM Presentation |
| Cost: | $30 |
| Dinner Choices: | Baked Salmon w/ Hollandaise Sauce Grilled chicken w/ bread stuffing Grilled Vegetable Cannelloni |
NOTICE TO STUDENTS AND FACULTY: Full-time students with valid ID may attend dinner meetings at half-price. Faculty members at colleges and universities are urged to bring one or more students to the meeting. If they do, they also can attend at half-price.