The Chromatography Forum

of Delaware Valley

For Reservations: Please register below by clicking "Register Now" where you can pay by PayPal. You can also register by e-mailing Sheree Gold at sheree@cfdv.org indicating you name, employer and work telephone. Alternatively, you can telephone Ms. Gold at 610-742-4981 and provide this same information. Please note that “no shows” will be billed for the dinner.

Inclement Weather Cancellation Policy: Cancellations will be accepted up until 12:00 pm (noon)  on the day of the meeting (January 23rd).

Generic Cancellation Policy: Cancellations will be accepted up until 4pm on January 22nd.

Abstract: Improved large-molecule separations are achieved when sample sizes are correctly matched with column pore diameter. During large-molecule separations, overlap of size- exclusion chromatography (SEC) with phase interaction modes (such as reversed- phase chromatography [RPC], normal-phase chromatography [NPC] and hydrophilic interaction chromatography [HILIC]) can be difficult to avoid. Data from this laboratory and others suggest that the rotational diameter of target analytes should be no larger than 10% of the mean pore diameter of the column packing particles to minimize pore crowding, maintain adequate retention and avoid significant loss of efficiency and resolution. This presentation will show examples of how increasing pore diameter improves separation and describe how optimum column pore size for separating large molecules can be identified initially to avoid a trial and error process.