The Chromatography Forum

of Delaware Valley

HALF-DAY COURSE - BREAKFAST AND LUNCH INCLUDED

FULL SCHEDULE TO BE POSTED SOON

Course attendees will learn about principles of chromatographic modes including reversed-phase, hydrophilic interaction, ion-exchange and size exclusion chromatography. Special emphasis will be given to reversed phase mode. Couse attendees will use MS Excel spreadsheets prepared to visualize chromatograms. Attendees will be able to change parameters such as particle size, column length, separation condition, extra column dispersion and others and instantly observe the effects in chromatographic separation. 

Topics: 

1. Principles of chromatographic separation, isocratic retention versus gradient 
2. Retention, resolution, peak capacity, band broadening, extra column dispersion 
3. Chromatographic behavior of small molecules versus biopolymers
4. Approaches to method development and method transfer.
5. SPE versus LC
6. Chromatographic artifacts of large volume injection, incompatible solvents, etc.

WHO SHOULD ATTEND
Chromatography practitioners, lab personal, students, casual LC users who are interested in deeper understanding of chromatographic principles. The attendees should bring their own laptop computers with installed MS Excel. The attendees will be provided with excel sheets based chromatographic simulators. They will be able to perform their own in-silico chromatographic experiments.