The Chromatography Forum

of Delaware Valley

Abstact:

While supercritical fluid chromatography (SFC) has been proved to be one of the most effective technologies in chiral separation, chiral separation of free amino acids and primary amines by SFC is still somewhat challenging because of the polar nature of these analytes. Crown ether-based CSPs are effective for the resolution of racemic compounds containing a primary amino group. This presentation will cover applications of chiral separation on crown ether column Crownpak CR-I(+) by SFC. The examples demonstrate that Crownpak CR-I(+) is a very useful column in chiral separation of free amino acids and primary amines in pharmaceutical industry and now a “go to” column for us when trying to separate these tricky polar racemates.

Speaker bio:

Lei Yue is a Senior Scientist II in the Analytics & Separations Department at Novartis Institutes for Biomedical Research in Cambridge, MA, where she has worked for the past two years. Lei graduated from Sichuan University in China with Master degree in Medicine Chemistry. She began her career with Daicel Chiral Technologies (China) in 2007 and then joined Chiral Technologies (US) in 2014. While working in Daicel Chiral Technologies, she mainly focused on chiral method development for analytical and preparative separation.  Lei moved to Boston area for family reason in 2017. Prior to joining Novartis, she worked for Averica Discovery and Amgen.